Journal: Cell reports
Article Title: Anti-dengue virus antibodies that elicit complement-mediated lysis of Zika virion correlate with protection from severe dengue disease.
doi: 10.1016/j.celrep.2025.115613
Figure Lengend Snippet: Figure 2. Complement-mediated virolysis with ZIKV-cross-reactive antibodies is strongly associated with protection from subsequent DHF/ DSS and symptomatic DENV infection (A–D) Pre-infection plasma samples prior to DF (green; n = 31) versus DHF/DSS (gold; n = 33) were incubated with mature DENV2, mature DENV3, ZIKV, and YFV virions. (A and B) Left: 1:10 dilution of plasma; right: 1:30 dilution of plasma. (E) Pre-inapparent (blue circles; n = 30) and pre-symptomatic (orange circles; n = 29) secondary DENV3 infection plasma samples were incubated with ZIKV virions. After incubating virions with plasma samples in the presence of guinea pig complement at 37◦C followed by one freeze-thaw cycle, viral RNA of unstable virions were digested with RNase A, while intact RNA was extracted from virolysis-resistant virions. Dengue-naive plasma samples were used as negative controls and set to the maximum amount of detectable RNA (lack of virolysis) in the assay. Virion lysis was quantified by measuring viral RNA via RT-qPCR (to measure the number of genome copies) and calculated as the percentage (%) of virion lysis as described in the STAR Methods. Shown are the median per centage of virolysis (middle line), 25th to 75th percentile (box), and 5th to 95th percentile (whiskers), as well as the raw data (points). Asterisks indicate Benjamini- Hochberg-adjusted p values for Mann-Whitney U tests, with false discovery rate (FDR) correction applied (****p < 0.0001, and ns, non-significant).
Article Snippet: Standard curves were prepared using seven 10-fold dilutions of a DENV2 8891 synthetic fragment, DENV3 UNC3009 in vitro transcribed RNA, ZIKV RNA standard (ATCC VR3252SD) or YFV synthetic RNA standard (ATCC VR-3253SD).
Techniques: Infection, Clinical Proteomics, Incubation, Lysis, Quantitative RT-PCR, MANN-WHITNEY